Z-Project

Interface

Input Video

Any videos supported by ffmpeg. This includes most video formats in existence. Notably, one exclusion: AVI videos saved from ImageJ with compression set to "None" (codec rawvideo) will not work.

Open Frame

Opens the first frame of the video as a preview. Can be used to set the ROI in functions that require it. Ignores the file's EXIF rotation data.

Output Image

The path to save the operation's image output. Tiff is the recommended format, although all formats supported by OpenCV imwrite are supported.

Save Output

Choose whether to save the output to the specified output path. If left unchecked, only displays the output.

Invert before operation

Invert each frame before performing the operation. Done as a bitwise NOT operation.

Processing Mode

The operations available mimic ImageJ's built-in Image → Stacks → Z project operations:

• Darkest (Min)
• Brightest (Max)
• Average
• Sum (saved as 32-bit)

Start and End frame

Specify the frame number to begin and end the processing.

The selection is inclusive, meaning both the start and end frames will be processed.

Frame numbers are one-indexed (the first frame has a frame number equal to 1).

To process the video all the way to its final frame, enter 0 in the 'End frame' field.

Preview

Shows a preview of the function, using the first 10 frames ahead of the Start frame set by the user.