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Plot Spots

Extract spots position data from a given Trackmate file. Outputs a scatter plot and a results table.

Results Table Output

Exporting data from imageJ's result tables to the default CSV option can led to problems related to decimal case separators. If you found this error, we recommend the usage of Excel Functions, a plugin that allows trouble-free data exports to xlsx format.

Interface

Interface image

XML File

Two possible files can be used here:

  1. Trackmate's whole save file
    • Ideal option overall.
    • Mandatory if you only have spots and no tracks. In this case, spots will be considered as-is, without fixing missing spots.
    • If there are any tracks, all spots outside them will get filtered out.
  2. Output of trackmate's Export tracks to XML file export action.
    • Only spots in tracks get saved in this file, so only them will be considered.

Source Video

The source video used for tracking. Ideally not the processed one, so you can set the ROIs better.

Output

  • Scatter Chart: Simple chart to represent spot positions. Scatter Chart image

How to reproduce this?